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Optical coherence hyperspectral microscopy with a single supercontinuum light source
Author(s) -
Chen Wei,
Chen Zhongjiang,
Xing Da
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000491
Subject(s) - supercontinuum , hyperspectral imaging , optical coherence tomography , microscopy , optics , optical microscope , materials science , biomedical engineering , microscope , coherence (philosophical gambling strategy) , medicine , computer science , optical fiber , physics , artificial intelligence , scanning electron microscope , quantum mechanics , photonic crystal fiber
In the paper, we have developed an optical coherence hyperspectral microscopy with a single supercontinuum light source. The microscopy consists of optical coherence tomography (OCT) and hyperspectral imaging (HSI), which can visualize the structural and functional characteristics of biological tissues. The 500 to 700 nm band is selected for HSI and OCT imaging, where HSI enables imaging of oxygen saturation and hemoglobin (Hb) content, while OCT acquires structural characteristics to assess the morphology of biological tissues. The system performance of the optical coherence hyperspectral microscopy is verified by normal mice ears, and the practical applications of the microscopy is further performed in 4T1 and inflammation Balb/c mice ears in vivo. The experimental results demonstrate that the microscopy has potential to provide complementary information for clinical applications.