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Exploring a possibility of using Raman spectroscopy for detection of Lyme disease
Author(s) -
Farber Charles,
Morey Rohini,
Krimmer Mark,
Kurouski Dmitry,
Rogovskyy Artem S.
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000477
Subject(s) - lyme disease , borrelia burgdorferi , serology , sensu stricto , virology , biology , immunology , microbiology and biotechnology , antibody , zoology
Lyme disease (LD), one of the most prevalent tick‐borne diseases in the United States (US), is caused by Borreliella burgdorferi sensu stricto ( Bb ). To date, in the US, LD diagnostics is primarily based on validated two‐tiered serological testing, which overall exhibits low sensitivity among other drawbacks. In the present study, a potential of Raman spectroscopy (RS) to detect Bb infection in mice has been explored. For that, C3H mice were infected with wild‐type Bb strains, 297, B31, or B31‐derived mutant, ∆ vlsE . Blood samples taken prior to and post Bb infection were subjected to RS. The data demonstrated that RS did not directly detect Bb spirochetes in blood, but rather sensed biochemical changes associated with Bb infection. Despite Bb infection‐associated blood changes detectable by RS were very limited, the partial least square discriminant analysis showed that the average true positive rates were 86% for 297 and 89% for B31 and ∆ vlsE .