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Multiphoton tomography in differentiation of morphological and molecular subtypes of breast cancer: A quantitative analysis
Author(s) -
Gubarkova Ekaterina V.,
Elagin Vadim V.,
Dudenkova Varvara V.,
Kuznetsov Sergey S.,
Karabut Maria M.,
Potapov Arseny L.,
Vorontsov Dmitry A.,
Vorontsov Alexey Y.,
Sirotkina Marina A.,
Zagaynova Elena V.,
Gladkova Natalia D.
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000471
Subject(s) - breast cancer , elastin , human breast , pathology , extracellular matrix , cancer , medicine , materials science , biomedical engineering , chemistry , biochemistry
Abstract In this study multiphoton tomography, based on second harmonic generation (SHG), and two‐photon‐excited fluorescence (TPEF) was used to visualize both the extracellular matrix and tumor cells in different morphological and molecular subtypes of human breast cancer. It was shown, that quantified assessment of the SHG based imaging data has great potential to reveal differences of collagen quantity, organization and uniformity in both low‐ and highly‐ aggressive invasive breast cancers. The values of quantity and uniformity of the collagen fibers distribution were significantly higher in low‐aggressive breast cancer compared to the highly‐aggressive subtypes, while the value representing collagen organization was lower in the former type. Additionally, it was shown, that TPEF detection of elastin fibers and amyloid protein may be used as a biomarker of detection the low‐aggressive breast cancer subtype. Thus, TPEF/SHG imaging offers the possibility of becoming a useful tool for the rapid diagnosis of various subtypes of breast cancer during biopsy as well as for the intraoperative determinination of tumor‐positive resection margins.