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Indocyanine green‐enhanced multimodal photoacoustic microscopy and optical coherence tomography molecular imaging of choroidal neovascularization
Author(s) -
Nguyen Van Phuc,
Folz Jeff,
Li Yanxiu,
Henry Jessica,
Zhang Wei,
Qian Thomas,
Wang Xueding,
Paulus Yannis M.
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000458
Subject(s) - indocyanine green , choroidal neovascularization , optical coherence tomography , neovascularization , molecular imaging , contrast (vision) , confocal microscopy , microscopy , biomedical engineering , medicine , pathology , ophthalmology , visual acuity , cancer research , optics , angiogenesis , in vivo , microbiology and biotechnology , biology , physics
Photoacoustic microscopy (PAM) has great potential for visualization of the microvasculature with high spatial resolution and contrast. Early detection and differentiation of newly developed blood vessels named choroidal neovascularization (CNV) from normal vasculature remains a challenge in ophthalmology. Exogenous contrast agents can assist with improving PAM sensitivity, leading to differentiation of CNV. Here, an FDA‐approved indocyanine green (ICG) was utilized as a PAM contrast agent. ICG was conjugated with RGD peptides, allowing the ICG to bind to the integrin expressed in CNV. Molecular PAM imaging showed that ICG‐RGD can target CNV for up to 5 days post intravenous administration in living rabbits with a model of CNV. The PAM image sensitivity and image contrast were significantly enhanced by 15‐fold at 24 h post‐injection. Overall, the presented approach demonstrates the possibility of targeted ICG to be employed in PAM molecular imaging, allowing more precise evaluation of neovascularization.