Premium
Fluorescence lifetime metabolic mapping of hypoxia‐induced damage in pancreatic pseudo‐islets
Author(s) -
Zbinden Aline,
Carvajal Berrio Daniel A.,
Urbanczyk Max,
Layland Shan L.,
Bosch Mariella,
Fliri Sandro,
Lu Chuanen,
Jeyagaran Abiramy,
Loskill Peter,
Duffy Garry P.,
SchenkeLayland Katja
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000375
Subject(s) - islet , transplantation , pancreatic islets , pancreas , population , diabetes mellitus , biology , medicine , endocrinology , environmental health
Abstract Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin‐secreting β‐cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stressed by a hypoxic environment and immune reactions that cause poor engraftment and graft failure. The current standards to assess islet quality before transplantation are destructive procedures, performed on a small islet population that does not reflect the heterogeneity of large isolated islet batches. In this study, we incorporated fluorescence lifetime imaging microscopy (FLIM) into a pancreas‐on‐chip system to establish a protocol to noninvasively assess the viability and functionality of pancreatic β‐cells in a three‐dimensional in vitro model (= pseudo‐islets). We demonstrate how (pre‐) hypoxic β‐cell‐composed pseudo‐islets can be discriminated from healthy functional pseudo‐islets according to their FLIM‐based metabolic profiles. The use of FLIM during the pretransplantation pancreatic islet selection process has the potential to improve the outcome of β‐cell islet transplantation.