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High‐fluence light emitting diode‐red light inhibits cell cycle progression in human dermal fibroblasts
Author(s) -
Masub Natasha,
Austin Evan,
Huang Alisen,
Jagdeo Jared
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000359
Subject(s) - fibroblast , microbiology and biotechnology , cell cycle , cell cycle checkpoint , cancer research , intense pulsed light , cell growth , chemistry , biology , cell , biochemistry , in vitro , genetics
Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high‐fluence light emitting diode‐generated red light (LED‐RL) is an alternative therapeutic that is safe, non‐invasive, and accessible. We previously demonstrated LED‐RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED‐RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED‐RL on human dermal fibroblast cell cycle progression. We demonstrate that LED‐RL at 640 J/cm 2 induced significant arrest of cells in G 0 /G 1 compared to temperature‐matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED‐RL may exert its anti‐proliferative effect on fibroblasts by inducing G 0 /G 1 arrest. Further, this study provides insight into the molecular mechanism underlying LED‐RL as an anti‐fibrotic therapeutic.