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Photobiomodulation and estrogen stabilize mitochondrial membrane potential in angiotensin– II challenged porcine aortic smooth muscle cells
Author(s) -
Gavish Lilach,
Gilon Dan,
Beeri Ronen,
Zuckerman Ayelet,
Nachman Dean,
Gertz S. David
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000329
Subject(s) - angiotensin ii , estrogen , mitochondrion , contraction (grammar) , medicine , endocrinology , chemistry , biology , microbiology and biotechnology , receptor
Abstract Rupture of Abdominal aortic aneurysm (AAA) is among the 15 leading causes of death after age 65. Using high frequency ultrasound, we showed that photobiomodulation (PBM) prevents formation and progression of AAA in the angiotensin‐II (Ang‐II)‐infused, apolipoprotein‐e‐deficient mouse model. In the current study we report that while challenge of porcine aortic Smooth Muscle Cells (SMCs) with Ang‐II (1 μM) resulted in a marked decay in mitochondrial membrane potential (MitMP) vs non‐challenged cells, treatment with PBM (continuous diode laser, 780 nm, 6.7 mW/cm 2 , 5 minutes, 2 J/cm 2 ) or pre‐incubation with estrogen (50 nM, 1 hour) significantly attenuated this deterioration in MitMP. We also report that PBM and estrogen markedly affected porcine aortic SMC contraction and modified mitochondrial dispersion reflecting important influence on SMC function. These studies provide strong evidence of the important underlying role of mitochondria in the preventive effect of PBM on formation and progression of AAA and its reduced incidence and delayed onset in women.