Premium
3‐photon microscopy of myelin in mouse digital skin excited at the 1700‐nm window
Author(s) -
He Chen,
Deng Xiangquan,
Pan Yi,
Tong Shen,
Kang Jiayu,
Li Jia,
Qiu Ping,
Wang Ke
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000321
Subject(s) - myelin , two photon excitation microscopy , microscopy , fluorescence microscope , in vivo , biophysics , excited state , materials science , chemistry , anatomy , fluorescence , biology , optics , central nervous system , neuroscience , physics , microbiology and biotechnology , nuclear physics
Myelin is a key component of the peripheral nervous system, whose structure anomaly in the digital skin is implicated in neuropathy. Here we demonstrate an in vivo labeling and imaging technique, capable of visualizing myelin sheaths deep in the mouse digital skin. Through material characterization, we verify that 3‐photon fluorescence (3PF) can be generated from a commonly used dye‐ FluoroMyelin Red for labeling myelin, excited at the 1700‐nm window. Topical injection of FluoroMyelin Red in the mouse digit leads to bright labeling of myelin sheaths. Harnessing the deep‐penetration capability of 3‐photon microscopy excited at the 1700‐nm window, we demonstrate that 3PF imaging of FluoroMyelin Red‐labeled myelin sheaths in the mouse digit in vivo can be achieved to a depth 340 μm below the skin surface, revealing both branching bundle of and individual myelin sheaths.