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Optimal hyperosmotic agents for tissue immersion optical clearing in terahertz biophotonics
Author(s) -
Musina Guzel R.,
Dolganova Iri.,
Chernomyrdin Nikita V.,
Gavdush Arsenii A.,
Ulitko Vladislav E.,
Cherkasova Olga P.,
Tuchina Daria K.,
Nikitin Pavel V.,
Alekseeva Anna I.,
Bal Natalia V.,
Komandin Gennady A.,
Kurlov Vladimir N.,
Tuchin Valery V.,
Zaytsev Kirill I.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000297
Subject(s) - biophotonics , terahertz radiation , materials science , spectroscopy , terahertz spectroscopy and technology , optics , penetration depth , opacity , optoelectronics , physics , photonics , quantum mechanics
Abstract In this work, a thorough analysis of hyperosmotic agents for the immersion optical clearing (IOC) in terahertz (THz) range was performed. It was aimed at the selection of agents for the efficient enhancement of penetration depth of THz waves into biological tissues. Pulsed spectroscopy in the frequency range of 0.1 to 2.5 THz was applied for investigation of the optical properties of common IOC agents. Using the collimated transmission spectroscopy in visible range, binary diffusion coefficients of tissue water and agent in ex vivo rat brain tissue were measured. IOC agents were objectively compared using two‐dimensional nomogram, accounting for their THz‐wave absorption coefficients and binary diffusion coefficients. The results of this study demonstrate an interplay between the penetration depth enhancement and the diffusion rate and allow for pointing out glycerol as an optimal agent among the considered ones for particular applications in THz biophotonics.