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Physicochemical damage and early‐stage biological response to X‐ray radiation studied in prostate cancer cells by Raman spectroscopy
Author(s) -
Roman Maciej,
Wrobel Tomasz P.,
Panek Agnieszka,
Paluszkiewicz Czeslawa,
Kwiatek Wojciech M.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000252
Subject(s) - ionizing radiation , prostate cancer , raman spectroscopy , irradiation , partial least squares regression , cancer , chemistry , cancer cell , biophysics , biology , genetics , optics , statistics , physics , mathematics , nuclear physics
Exposure to ionizing radiation significantly affects biochemistry of cancer cells. The effect of irradiation can be divided into two stages, that is, the physicochemical stage and the biological response. Both effects induce different biochemical changes in the cells and should be analyzed as two separate phenomena. Thus, in the current study, Raman spectroscopy of prostate cancer cells fixed before (the physicochemical damage model) and just after (the biological response model) irradiation was undertaken to compare biochemical composition of irradiated cancer cells at both stages. Spectroscopic analysis of the cells was performed separately for cytoplasmic and nuclear regions. Biochemical changes of irradiated cells were analyzed using partial least squares regression (PLSR) method on the basis of the collected Raman spectra. Regression coefficients were therefore used to describe differences and similarities between biochemical composition of cancer cells undergoing the physicochemical stage and biological response. Additionally, PLSR models of both phenomena were compared for linear dose‐dependence and a cross prediction.