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Cortex‐wide microcirculation mapping with ultrafast large‐field multifocal illumination microscopy
Author(s) -
Chen Zhenyue,
Zhou Quanyu,
Rebling Johannes,
Razansky Daniel
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000198
Subject(s) - temporal resolution , microscopy , image resolution , frame rate , optics , biomedical engineering , neuroimaging , raster scan , field of view , microcirculation , materials science , physics , computer vision , computer science , neuroscience , biology , medicine , radiology
The recently introduced large‐field multifocal illumination (LMI) fluorescence microscopy technique opened new possibilities for transcranial observations of mouse brain dynamics with a unique combination of capillary level resolution and centimeter‐scale field‐of‐view (FOV). Here we report on a new acceleration scheme for LMI based on raster scan of a lattice pattern combined with a parallel camera exposure scheme, which attains 200 Hz frame rate over 12 × 12 mm 2 FOV with 7.5 μm spatial resolution. We demonstrate real‐time transcranial in vivo tracking of particles and imaging of microcirculation across the entire mouse cortex, thus corroborating the superb spatiotemporal resolution performance of LMI unattainable with other techniques. Potential applications include investigations into cerebrovascular function, cell tracking, as well as large‐scale functional neuroimaging.