Premium
Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination
Author(s) -
Kassab Giulia,
Cheburkanov Vsevolod,
Willis Jace,
Moule Madeleine G.,
Kurachi Cristina,
Yakovlev Vladislav,
Cirillo Jeffrey D.,
Bagnato Vanderlei S.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000176
Subject(s) - photodynamic therapy , photobleaching , indocyanine green , staphylococcus aureus , photosensitizer , toxicity , antimicrobial , microbiology and biotechnology , pneumonia , medicine , chemistry , bacteria , biology , fluorescence , surgery , photochemistry , physics , organic chemistry , quantum mechanics , genetics
Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high‐mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing of Staphylococcus aureus (of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused by S. aureus and other important pathogens.