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Multisite cell‐ and neural‐dynamics‐resolving deep brain imaging in freely moving mice with implanted reconnectable fiber bundles
Author(s) -
Pochechuev Matvey S.,
Solotenkov Maxim A.,
Fedotov Ilya V.,
Ivashkina Olga I.,
Anokhin Konstantin V.,
Zheltikov Aleksei M.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000081
Subject(s) - neocortex , calcium imaging , neuroscience , fiber bundle , fluorescence lifetime imaging microscopy , branching (polymer chemistry) , fluorescence , fiber , hippocampus , dynamics (music) , calcium , biomedical engineering , biophysics , biology , materials science , physics , optics , medicine , acoustics , metallurgy , composite material
We demonstrate a reconnectable implantable ultraslim fiber‐optic microendoscope that integrates a branching fiber bundle (BFB) with gradient‐index fiber lenses, enabling a simultaneous fluorescence imaging of individual cells in distinctly separate brain regions, including brain structures as distant as the neocortex and hippocampus. We show that fluorescence images of individual calcium‐indicator‐expressing neurons in the brain of freely moving transgenic mice can be recorded, via the implanted BFB probe, in parallel with time‐ and cell‐resolved traces of calcium signaling, thus enabling correlated circuit‐dynamics studies at ‐multiple sites within the brain of freely moving animals.