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Computed tomography for in vivo deep over‐1000 nm near‐infrared fluorescence imaging
Author(s) -
Umezawa Masakazu,
Sera Toshihiro,
Yokota Hideo,
Takematsu Maho,
Morita Masahiko,
Yeroslavsky Gil,
Kamimura Masao,
Soga Kohei
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000071
Subject(s) - materials science , fluorescence , fluorophore , optics , near infrared spectroscopy , fluorescence lifetime imaging microscopy , preclinical imaging , biomedical engineering , in vivo , physics , medicine , microbiology and biotechnology , biology
This study aims to develop a novel cross‐sectional imaging of fluorescence in over‐1000 nm near‐infrared (OTN‐NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN‐NIR fluorophore, NaGdF 4 co‐doped with Yb 3+ and Ho 3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample‐air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN‐NIR fluorophores on the cross‐sectional images using a CT technique. This is promising as a novel three‐dimensional imaging technique for OTN‐NIR fluorescent image projections of small animals captured from multiple angles.