Premium
Long‐term and in vivo assessment of Aβ protein‐induced brain atrophy in a zebrafish model by optical coherence tomography
Author(s) -
Lin Yanping,
Chen Tingru,
Mao Guangjuan,
Qiu Ting,
Lan Yintao,
Xiang Xiang,
Huang Jie,
Huang Jing,
Lu Ting,
Gan Shuqi,
Sun XiangDong,
Zhang Jian
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000067
Subject(s) - zebrafish , in vivo , atrophy , optical coherence tomography , neurotoxicity , pathology , preclinical imaging , medicine , biology , ophthalmology , toxicity , biochemistry , microbiology and biotechnology , gene
In this study, a neurotoxicity model of zebrafish induced by amyloid beta (Aβ) protein was developed and evaluated in vivo by optical coherence tomography (OCT). Aβ protein and phosphate buffer saline (PBS) were separately injected into the head of two groups of adult zebrafish (n = 6 per group). Congo‐red staining results confirmed that Aβ protein had penetrated into brain tissue. All zebrafish were imaged with OCT on the 0th, 5th, 10th, 15th and 20th day postinjection. OCT images showed that PBS is not toxic to brain tissue. However, significant brain atrophy could be seen in the OCT images of zebrafish injected with Aβ‐protein that was verified by histological consequences. In addition, zebrafish in the model group showed memory decline in behavioral tests. This study verified the feasibility of in vivo long‐term assessment of Aβ protein‐induced brain atrophy in adult zebrafish by OCT that has great potential to be applied in the neurological diseases research.