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Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser‐fabricated micro through‐holes
Author(s) -
Takayama Izumi,
Kondo Naonari,
Kalies Stefan,
Heisterkamp Alexander,
Terakawa Mitsuhiro
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000037
Subject(s) - c2c12 , materials science , adhesion , femtosecond , cell adhesion , laser , substrate (aquarium) , tissue engineering , nanotechnology , composite material , myocyte , biomedical engineering , optics , microbiology and biotechnology , medicine , oceanography , physics , myogenesis , geology , biology
Abstract Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through‐holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2‐C2C12 and UT‐C2C12 cells were seeded on the films with micro through‐holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through‐holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through‐holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.