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Multicomposite super‐resolution microscopy: Enhanced Airyscan resolution with radial fluctuation and sample expansions
Author(s) -
Wang Baoju,
Yao Longfang,
Jing Yueyue,
Fei Yiyan,
Bai Qianming,
Mi Lan,
Ma Jiong
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201960211
Subject(s) - resolution (logic) , microscopy , image resolution , superresolution , super resolution microscopy , optics , sample (material) , microscope , diffraction , materials science , human epidermal growth factor receptor 2 , chemistry , physics , scanning confocal electron microscopy , computer science , breast cancer , artificial intelligence , biology , cancer , image (mathematics) , chromatography , genetics
Either modulated illumination or temporal fluctuation analysis can assist super‐resolution techniques in overcoming the diffraction limit of conventional optical microscopy. As they are not contradictory to each other, an effective combination of spatial and temporal super‐resolution mechanisms would further improve the resolution of fluorescent images. Here, a super‐resolution imaging method called fluctuation‐enhanced Airyscan technology (FEAST) is proposed, which achieves ~40 nm lateral imaging resolution and is useful for a range of fluorescent proteins and organic dyes. It was demonstrated not only to obtain different subcellular super‐resolution images, but also to improve the accuracy of counting the average human epidermal growth factor receptor 2 (HER2) copy number for diagnosis in breast cancer. Furthermore, the combination of FEAST and sample expansion microscopy (Ex‐FEAST) improves the lateral resolution to ~26 nm.