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Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy
Author(s) -
Golaraei Ahmad,
Kontenis Lukas,
Karunendiran Abiramy,
Stewart Bryan A.,
Barzda Virginijus
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201960167
Subject(s) - microscopy , single shot , optics , one shot , shot (pellet) , materials science , harmonic , physics , acoustics , engineering , mechanical engineering , metallurgy
Polarization‐resolved second‐harmonic generation (P‐SHG) microscopy is a technique capable of characterizing nonlinear optical properties of noncentrosymmetric biomaterials by extracting the nonlinear susceptibility tensor components ratio χ zzz 2 ′ / χ zxx 2 ′ , with z ‐axis parallel and x ‐axis perpendicular to the C 6 symmetry axis of molecular fiber, such as a myofibril or a collagen fiber. In this paper, we present two P‐SHG techniques based on incoming and outgoing circular polarization states for a fast extraction of χ zzz 2 ′ / χ zxx 2 ′ : A dual‐shot configuration where the SHG circular anisotropy generated using incident right‐ and left‐handed circularly‐polarized light is measured; and a single‐shot configuration for which the SHG circular anisotropy is measured using only one incident circular polarization state. These techniques are used to extract the χ zzz 2 ′ / χ zxx 2 ′of myosin fibrils in the body wall muscles of Drosophila melanogaster larva. The results are in good agreement with values obtained from the double Stokes‐Mueller polarimetry. The dual‐ and single‐shot circular anisotropy measurements can be used for fast imaging that is independent of the in‐plane orientation of the sample. They can be used for imaging of contracting muscles, or for high throughput imaging of large sample areas.