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Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues
Author(s) -
Darby Jack R. T.,
Sorvina Alexandra,
Bader Christie A.,
Lock Mitchell C.,
Soo Jia Yin,
Holman Stacey L.,
Seed Mike,
Kuchel Tim,
Brooks Douglas A.,
Plush Sally E.,
Morrison Janna L.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201960085
Subject(s) - skeletal muscle , adipose tissue , glycolysis , oxidative phosphorylation , fetus , biology , metabolism , redox , biochemistry , endocrinology , chemistry , medicine , pregnancy , genetics , organic chemistry
The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.