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Effect of novel porphyrazine photosensitizers on normal and tumor brain cells
Author(s) -
Mishchenko Tatiana A.,
Turubanova Victoria D.,
Mitroshina Elena V.,
Alzeibak Razan,
Peskova Ni.,
Lermontova Svetlana A.,
Klapshina Larisa G.,
Balalaeva Irina V.,
Vedunova Maria V.,
Krysko Dmitri V.
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201960077
Subject(s) - glioma , photosensitizer , photodynamic therapy , cytotoxicity , chemistry , cancer research , internalization , cancer cell , in vitro , porphyrazine , biophysics , microbiology and biotechnology , cancer , biochemistry , cell , biology , medicine , photochemistry , organic chemistry
Photodynamic therapy (PDT) is a clinically approved procedure for targeting tumor cells. Though several different photosensitizers have been developed, there is still much demand for novel photosensitizers with improved properties. In this study we aim to characterize the accumulation, localization and dark cytotoxicity of the novel photosensitizers developed in‐house derivatives of porphyrazines ( pz I‐IV) in primary murine neuronal cells, as well as to identify the concentrations at which pz still effectively induces death in glioma cells yet is nontoxic to nontransformed cells. The study shows that incubation of primary neuronal and glioma cells with pz I‐IV leads to their accumulation in both types of cells, but their rates of internalization, subcellular localization and dark toxicity differ significantly. Pz II was the most promising photosensitizer. It efficiently killed glioma cells while remaining nontoxic to primary neuronal cells. This opens up the possibility of evaluating pz II for experimental PDT for glioma.