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Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles
Author(s) -
Stepula Elzbieta,
König Matthias,
Wang XinPing,
Levermann Janina,
Schimming Tobias,
KasimirBauer Sabine,
Schilling Bastian,
Schlücker Sebastian
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201960034
Subject(s) - core (optical fiber) , microscopy , satellite , nanotechnology , nanoparticle , cancer , fluorescence microscope , cancer cell , chemistry , materials science , biology , physics , medicine , pathology , fluorescence , optics , genetics , astronomy , composite material
Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies.