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Morphological and functional characteristics of aging kidneys based on two‐photon microscopy in vivo
Author(s) -
Wu Shulian,
Guo Hengchang,
Horng Hannah,
Liu Yi,
Li Hui,
Daneshpajouhnejad Parnaz,
Rosenberg Avi,
Albanese Christopher,
Ranjit Suman,
Andrews Peter M.,
Levi Moshe,
Tang Qinggong,
Chen Yu
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900246
Subject(s) - in vivo , kidney , renal function , kidney disease , pathology , transplantation , dextran , biology , chemistry , medicine , endocrinology , biochemistry , microbiology and biotechnology
Age‐related kidney disease, which is chronic and naturally occurring, is a general term for a set of heterogeneous disorders affecting kidney structures and characterized by a decline in renal function. Age‐related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two‐photon microscopy was used to image kidney morphological and functional characteristics in an age‐related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high‐molecular weight (MW) 500‐kDa dextran‐fluorescein and low‐MW 10‐kDa dextran‐rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age‐related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and/or therapeutic effects of kidney drugs.