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Self‐assembly of lipid rafts revealed by fluorescence correlation spectroscopy in living breast cancer cells
Author(s) -
Dong Shiqing,
Tang Xiaoqiong,
Wang Jiao,
Zhang Zhenghong,
Chen Jianling,
Lin Yao,
Xie Shusen,
Wang Zhengchao,
Yang Hongqin
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900214
Subject(s) - lipid raft , fluorescence correlation spectroscopy , lipid microdomain , cholera toxin , chemistry , raft , biophysics , microbiology and biotechnology , lipid bilayer , lipid bilayer fusion , membrane , membrane biophysics , membrane fluidity , fluorescence microscope , biology , biochemistry , fluorescence , molecule , physics , organic chemistry , quantum mechanics , copolymer , polymer
Lipids and proteins in the plasma membrane are laterally heterogeneous and formalised as lipid rafts featuring unique biophysical properties. However, the self‐assembly mechanism of lipid raft cannot be revealed even its physical properties and components were determined in specific physiological processes. In this study, two‐photon generalised polarisation imaging and fluorescence correlation spectroscopy were used to study the fusion of lipid rafts through the membrane phase and the lateral diffusion of lipids in living breast cancer cells. A self‐assembly model of lipid rafts associated with lipid diffusion and membrane phase was proposed to demonstrate the lipid sorting ability of lipid rafts in the plasma membrane. The results showed that the increased proportion of slow subdiffusion of G M1 ‐binding cholera toxin B‐subunit (CT‐B) was accompanied with an increased liquid‐ordered domain during the β‐estradiol‐induced fusion of lipid rafts. And slow subdiffusion of CT‐B was vanished with the depletion of lipid rafts. Whereas the dialkylindocarbocyanine (DiIC 18 ) diffusion was not specifically regulated by lipid rafts. This study will open up a new insight for uncovering the self‐assembly of lipid rafts in specific pathophysiological processes.