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Quantitative investigation of vascular response to mesenteric venous thrombosis using large‐field‐of‐view photoacoustic microscopy
Author(s) -
Qin Wei,
Qi Weizhi,
Xi Lei
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900198
Subject(s) - mesenteric vein , photoacoustic imaging in biomedicine , superior mesenteric vein , thrombosis , medicine , vein , mesenteric arteries , radiology , biomedical engineering , cardiology , surgery , optics , portal vein , artery , physics
Mesenteric venous thrombosis (MVT) is one of major causes leading to severe mesenteric ischemia. Vascular network plays an important role during the occurrence and development of MVT. However, there lacks an appropriate imaging method, which features advanced volumetric resolving capability, superior sensitivity to hemoglobin, and ultra‐large field‐of‐view (FOV), to investigate vascular response of MVT. In this study, we developed and applied a large‐FOV optical resolution photoacoustic microscopy to quantify the vascular response during the entire course of two different MVT models in which we ligated the superior mesenteric vein and inferior mesenteric vein, respectively. Furthermore, we developed a quantitative algorithm to derive total vascular length, relative concentration of total hemoglobin and vascular density over the FOV to reveal different vascular responses in different MVT models.