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Quadrature demodulation in line‐scan focal modulation microscopy for imaging three‐dimensional zebrafish neural structure
Author(s) -
Shen Shuhao,
Wu Jian,
Chen Nanguang
Publication year - 2020
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900170
Subject(s) - demodulation , optics , microscopy , microscope , optical sectioning , light sheet fluorescence microscopy , image resolution , materials science , focus (optics) , fluorescence microscope , physics , computer science , scanning confocal electron microscopy , fluorescence , telecommunications , channel (broadcasting)
Visualizing biological processes in neuroscience requires in vivo functional imaging at single‐neuron resolution, high image acquisition speed and strong optical sectioning ability. However, due to light scattering of in tissue, very often conventional wide‐field fluorescence microscopes are unable to resolve cells in the presence of a strong out‐of‐focus background. Line‐scan focal modulation microscopy enables high temporal resolution and good optical sectioning ability at the same time. Here we demonstrate a quadrature demodulation method to extract the focal information with an extended frequency bandwidth and therefore higher spatial resolution. The performance of the demodulation scheme in line‐scan focal modulation microscope has been evaluated by performing imaging experiments with fluorescence beads and zebrafish neural structure. Reduced background, reduced artifacts and more detailed morphological information are evident in the obtained images.