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Quantitative assessment of microenvironment characteristics and metabolic activity in glioma via multiphoton microscopy
Author(s) -
Fang Na,
Wu Zanyi,
Wang Xingfu,
Lin Yuanxiang,
Li Lianhuang,
Huang Zufang,
Chen Yupeng,
Zheng Xianying,
Cai Shanshan,
Tu Haohua,
Kang Dezhi,
Chen Jianxin
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900136
Subject(s) - tumor microenvironment , glioma , angiogenesis , metabolic activity , cancer research , multiphoton fluorescence microscope , chemistry , extracellular matrix , in vivo , tumor progression , pathology , fluorescence microscope , medicine , biology , fluorescence , tumor cells , biochemistry , optics , physics , physiology , microbiology and biotechnology , gene
Tumor microenvironment and metabolic activity in gliomas are the important biomarkers to evaluate the progression of gliomas. Many evidences have suggested that the targeting of metabolic activity and tumor microenvironment simultaneously can be more effective to take the tumor therapy. Therefore, the noninvasive, accurate assessment of tumor microenvironment and metabolic activity is quite important in clinical practice. Multiphoton microscopy (MPM), based on two‐photon‐excited fluorescence and second harmonic generation was performed on unstained glioma tissues. With our combined image analysis approaches, our research findings indicate that MPM is able to qualitatively and quantitatively describe the microenvironment characteristics in gliomas, such as collage deposition in extracellular matrix, lymphocyte infiltration and tumor angiogenesis, etc. Meanwhile, the metabolic activity can also be quantitatively evaluated by optical redox ratio, NADH and FAD intensity. With the microendoscope and fiberscope are portable, MPM technique can be used to perform in‐vivo studies and clinical examinations in gliomas.