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Light‐emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology
Author(s) -
Choi SunHyang,
Chang SoYoung,
Biswas Raktim,
Chung PhilSang,
Mo Sangjoon,
Lee Min Young,
Ahn Jin Chul
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201900063
Subject(s) - viability assay , hsp27 , heat shock protein , microbiology and biotechnology , fibroblast , hsp70 , blot , biology , cell , chemistry , cell culture , biochemistry , gene , genetics
We evaluated changes in cell viability and morphology in response to low‐level light irradiation and underlying variations in the levels of heat shock proteins (HSPs). Human fibroblasts were irradiated with a light‐emitting diode (LED) array at 660 nm (50 mW for 15, 30, and 60 minutes). Cell viability and morphological changes were evaluated via epifluorescence analysis; we also assessed cell viability and length changes. The expression levels of adenosine triphosphate (ATP) and various HSPs (HSP27, 60, 70, and 90) were analyzed by immunohistochemical staining, Western blotting and microarray analysis. After LED irradiation, cellular viability and morphology changed. Of the several HSPs analyzed, the HSP90 level increased significantly, suggesting that this protein played roles in the morphological and cellular changes. Thus, low‐level irradiation triggered cellular changes mediated by increased HSP90 expression; this may explain why skin irradiation enhances wound‐healing.