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Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues
Author(s) -
Ayala Oscar D.,
Doster Ryan S.,
Manning Shan D.,
O'Brien Christine M.,
Aronoff David M.,
Gaddy Jennifer A.,
MahadevanJansen Anita
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800449
Subject(s) - ex vivo , chorioamnionitis , streptococcus agalactiae , neonatal sepsis , microbiology and biotechnology , group b , staphylococcus aureus , biology , streptococcus , fetus , medicine , in vivo , sepsis , bacteria , immunology , pregnancy , genetics
Streptococcus agalactiae , also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.