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Two‐dimensional correlation analysis of Raman microspectroscopy of subcellular interactions of drugs in vitro
Author(s) -
Byrne Hugh J.,
Bonnier Franck,
Farhane Zeineb
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800328
Subject(s) - nucleolus , chemistry , doxorubicin , raman spectroscopy , correlation , organelle , biophysics , drug response , subcellular localization , biological system , computational biology , biology , drug , biochemistry , physics , genetics , pharmacology , cytoplasm , mathematics , geometry , chemotherapy , optics
Two‐dimensional (2D) correlation analysis is explored to data mine the time evolution of the characteristic Raman microspectroscopic signatures of the subcellular responses of the nucleoli of human lung cancer cells to the uptake of doxorubicin. A simulated dataset of experimental control spectra, perturbed with systematically time‐dependent spectral changes, constituted by a short‐term response which represents the initial binding of the drug in the nucleolus, followed by a longer term response of the organelle metabolism, is used to validate the analysis protocol. Applying 2D correlation analysis, the in phase, synchronous correlation coefficients are seen to contain contributions of both response profiles, whereas they can be independently extracted from the out of phase, asynchronous correlation coefficients. The methodology is applied to experimental data of the uptake of doxorubicin in human lung cell lines to differentiate the signatures of chemical binding and subsequent cellular response.