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Dynamic visualization of the recovery of mouse hepatobiliary metabolism to acetaminophen‐overdose damage
Author(s) -
Li FengChieh,
Lee ShengLin,
Lin HungMing,
Lin ChihJu,
Wang ShengShun,
Lee YuYang,
Lo SuYen,
Sun TzuLin,
Chen WeiLiang,
Lo Wen,
Horton Nicholas,
Xu Chris,
Chiang ShuJen,
Chen YangFang,
Lee HsuanShu,
Dong ChenYuan
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800296
Subject(s) - acetaminophen , liver damage , metabolism , medicine , chemistry , pharmacology
Acetaminophen (APAP) overdose is one of the world's leading causes of drug‐induced hepatotoxicity. Although traditional methods such as histological imaging and biochemical assays have been successfully applied to evaluate the extent of APAP‐induced liver damage, detailed effect of how APAP overdose affect the recovery of hepatobiliary metabolism and is not completely understood. In this work, we used intravital multiphoton microscopy to image and quantify hepatobiliary metabolism of the probe 6‐carboxyfluorescein diacetate in APAP‐overdose mice. We analyzed hepatobiliary metabolism for up to 7 days following the overdose and found that the excretion of the probe molecule was the most rapid on Day 1 following APAP overdose and slowed down on Days 2 and 3. On Day 7, probe excretion capability has exceeded that of the normal mice, suggesting that newly regenerated hepatocytes have higher metabolic capabilities. Our approach may be further developed applied to studying drug‐induced hepatotoxicity in vivo.