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Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes
Author(s) -
Szafraniec Ewelina,
Kus Edyta,
Wislocka Adrianna,
Kukla Bozena,
Sierka Ewa,
Untereiner Valérie,
Sockalingum Ganesh D.,
Chlopicki Stefan,
Baranska Malgorzata
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800290
Subject(s) - nonalcoholic fatty liver disease , lipid droplet , chemistry , raman spectroscopy , lipid accumulation , medicine , fatty liver , endocrinology , microbiology and biotechnology , biology , biochemistry , disease , physics , optics
Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis, and LSECs dysfunction is involved in the development of nonalcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high‐fat diet. On NAFLD development, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo‐CoA desaturase‐1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging.