Premium
Low‐coherent optical diffraction tomography by angle‐scanning illumination
Author(s) -
Lee KyeoReh,
Shin Seungwoo,
Yaqoob Zahid,
So Peter T. C.,
Park YongKeun
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800289
Subject(s) - optics , diffraction , interference (communication) , interferometry , digital micromirror device , materials science , optical sectioning , tomography , physics , microscopy , computer science , computer network , channel (broadcasting)
Temporally low‐coherent optical diffraction tomography (ODT) is proposed and demonstrated based on angle‐scanning Mach‐Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, the full‐field interference of incoherent light is successfully maintained during every angle‐scanning sequences. Further, current ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are also discussed, such as the nondispersive assumption, optical sectioning capacity and illumination angle limitation. Using the proposed setup and reconstruction algorithms, low‐coherent ODT imaging of plastic microspheres, human red blood cells and rat pheochromocytoma cells is experimentally demonstrated.