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Large field of view correction by using conjugate adaptive optics with multiple guide stars
Author(s) -
Zhao Qi,
Shi Xin,
Zhu Xinpei,
Zheng Yao,
Wu Chenxue,
Tang Hengjie,
Hu Lejia,
Xue Ying,
Gong Wei,
Si Ke
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800225
Subject(s) - adaptive optics , wavefront , optics , field of view , computer science , physics , deformable mirror , stars , pupil , artificial intelligence , computer vision
Adaptive optics has been widely used in the optical microscopy to recover high‐resolution images deep into the sample. However, the corrected field of view (FOV) with a single correction is generally limited, which seriously restricts the imaging speed. In this article, we demonstrate a high‐speed wavefront correction method by using the conjugate adaptive optical correction with multiple guide stars (CAOMG) based on the coherent optical adaptive technique. The results show that the CAOMG method can greatly improve the corrected FOV. For 120‐μm‐thick mouse brain tissue, the corrected FOV can be improved up to ~243 times of the conventional pupil adaptive optics (PAO) without additional time consumption. Therefore, this study shows the potential of high‐speed imaging through scattering medium in biological science.