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Functional optical coherence tomography of neurovascular coupling interactions in the retina
Author(s) -
Son Taeyoon,
Alam Minhaj,
Toslak Devrim,
Wang Benquan,
Lu Yiming,
Yao Xincheng
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800089
Subject(s) - retina , optical coherence tomography , hemodynamics , neuroscience , ganglion cell layer , stimulus (psychology) , retinal , outer plexiform layer , haemodynamic response , ophthalmology , anatomy , medicine , biology , psychology , cardiology , heart rate , blood pressure , psychotherapist
Quantitative evaluation of retinal neurovascular coupling is essential for a better understanding of visual function and early detection of eye diseases. However, there is no established method to monitor coherent interactions between stimulus‐evoked neural activity and hemodynamic responses at high resolution. Here, we report a multimodal functional optical coherence tomography (OCT) imaging methodology to enable concurrent intrinsic optical signal (IOS) imaging of stimulus‐evoked neural activity and hemodynamic responses at capillary resolution. OCT angiography guided IOS analysis was used to separate neural‐IOS and hemodynamic‐IOS changes in the same retinal image sequence. Frequency flicker stimuli evoked neural‐IOS changes in the outer retina; that is, photoreceptor layer, first and then in the inner retina, including outer plexus layer (OPL), inner plexiform layer (IPL), and ganglion cell layer (GCL), which were followed by hemodynamic‐IOS changes primarily in the inner retina; that is, OPL, IPL, and GCL. Different time courses and signal magnitudes of hemodynamic‐IOS responses were observed in blood vessels with various diameters.