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Correlation of intracellular oxygen and cell metabolism by simultaneous PLIM of phosphorescent TLD1433 and FLIM of NAD(P)H
Author(s) -
Kalinina Sviatlana,
Breymayer Jasmin,
Reeß Kirsten,
Lilge Lothar,
Mandel Arkady,
Rück Angelika
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800085
Subject(s) - nad+ kinase , nicotinamide adenine dinucleotide , photosensitizer , biophysics , phosphorescence , fluorescence lifetime imaging microscopy , chemistry , oxygen , photodynamic therapy , redox , cellular respiration , singlet oxygen , reactive oxygen species , fluorescence , photochemistry , mitochondrion , biochemistry , biology , optics , physics , organic chemistry , enzyme
During photodynamic therapy (PDT), disruption of cell respiration and metabolic changes could be one of the first events. Photophysical characteristics of the photosensitizer (PS) and its specific redox potential define consumption of molecular oxygen followed by generation of reactive oxygen species. The potential PS TLD1433 is based on transition metal Ru(II) and possess an oxygen‐dependent luminescence. This enables the study of oxygen consumption by PS‐phosphorescence lifetime imaging (PLIM) and simultaneously changes the cellular metabolic state by nicotinamide adenine dinucleotide (NAD(P)H)‐fluorescence lifetime imaging (FLIM). Within this study, localization and cellular function of TLD1433 is investigated in bladder carcinoma cells using time‐resolved and confocal laser scanning microscopy. Simultaneous FLIM/PLIM of NAD(P)H and TLD1433 during PDT correlated oxygen consumption, redox state and cellular energy metabolism. Our investigations aimed to provide a personalized protocol in theranostic PDT procedures and demonstrate the potential use of TLD1433 PDT also under hypoxic conditions, which are otherwise difficult to treat.