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Measurement of flow‐mediated dilation of mouse femoral artery in vivo by optical coherence tomography
Author(s) -
Song Weiye,
Zhou Libo,
Kot Kevin L.,
Fan Huijie,
Han Jingyan,
Yi Ji
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800053
Subject(s) - optical coherence tomography , medicine , femoral artery , in vivo , blood flow , vasodilation , artery , nitric oxide , laser doppler velocimetry , tomography , biomedical engineering , cardiology , radiology , biology , microbiology and biotechnology
Flow‐mediated vasodilation (FMD) is used for assessment of vascular endothelial function in humans as a predictor of cardiovascular events. It has been challenging to carry it on preclinical murine models due to the diminutive size of the femoral artery. Here, we present a new approach to accurately measure the blood velocity and femoral artery diameters of mice by acquiring Doppler optical coherence tomography and optical coherence tomography angiography continuously within 1 single experimental scanning protocol. Using the 3‐dimensional imaging and new velocity algorithm, the measurement precision of diameter, blood flow, velocity and wall shear stress are improved to 0.91%, 11.0%, 10.7% and 14.0%, respectively. FMD of healthy mouse femoral artery measured by this method was 11.96% ± 0.98%, which was blunted to 5.69% ± 0.4% by intravenous administration of endothelial nitric oxide synthase inhibitor (L‐N G ‐Nitroarginine methyl ester), in agreement with that reported in the literature.