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Ultrafast cell edge detection by line‐scan time‐stretch microscopy
Author(s) -
Dai Bo,
He Lu,
Zheng Lulu,
Fu Yongfeng,
Wang Kaimin,
Sui Guodong,
Zhang Dawei,
Zhuang Songlin,
Wang Xu
Publication year - 2019
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800044
Subject(s) - edge detection , microscopy , materials science , enhanced data rates for gsm evolution , biomedical engineering , throughput , computer science , image processing , computer vision , optics , image (mathematics) , medicine , physics , telecommunications , wireless
Ultrafast time‐stretch imaging technique recently attracts an increasing interest for applications in cell classification due to high throughput and high sensitivity. A novel imaging modality of time‐stretch imaging technique for edge detection is proposed. Edge detection based on the directional derivative is realized using differential detection. As the image processing is mainly implemented in the physical layer, the computation complexity of edge extraction is significantly reduced. An imaging system for edge detection with the scan rate of 77.76 MHz is experimentally demonstrated. Resolution target is first measured to verify the feasibility of the edge extraction. Furthermore, various cells, including red blood cells, lung cancer cells and breast cancer cells, are detected. The edges of cancerous cells present in a completely different form. The imaging system for edge detection would be a good candidate for high‐throughput cell classification.