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Experimental study of the evanescent‐wave photonic sensors response in presence of molecular beacon conformational changes
Author(s) -
RuizTórtola Ángela,
PratsQuílez Francisco,
GonzálezLucas Daniel,
Bañuls MaríaJosé,
Maquieira Ángel,
Wheeler Guy,
Dalmay Tamas,
Griol Amadeu,
Hurtado Juan,
Bohlmann Helge,
Götzen Reiner,
GarcíaRupérez Jaime
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800030
Subject(s) - streptavidin , molecular beacon , photonics , materials science , conformational change , oligonucleotide , molecular binding , molecule , evanescent wave , nanotechnology , chemistry , biotin , optoelectronics , stereochemistry , biochemistry , dna , organic chemistry
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes—upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors—is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5′ termination. Those MBs incorporate a biotin moiety close to their 3′ termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure.