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Cerebral capillary flow imaging by wavelength‐division‐multiplexing swept‐source optical Doppler tomography
Author(s) -
Chen Wei,
Du Congwu,
Pan Yingtian
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201800004
Subject(s) - optical coherence tomography , optics , doppler effect , multiplexing , materials science , tomography , capillary action , preclinical imaging , biomedical engineering , physics , in vivo , medicine , telecommunications , computer science , microbiology and biotechnology , astronomy , composite material , biology
Swept‐source‐based optical coherence tomography (SS‐OCT) has demonstrated the unique advantages for fast imaging rate and long imaging distance; however, limited axial resolution and complex phase noises restrict swept‐source optical Doppler tomography (SS‐ODT) for quantitative capillary blood flow imaging in the deep cortices. Here, the wavelength‐dividing‐multiplexing optical Doppler tomography (WDM‐ODT) method that divides a single interferogram into multiple phase‐correlated interferograms is proposed to effectively enhance the sensitivity for cerebral capillary flow imaging. Both flow phantom and in vivo mouse brain imaging studies show that WDM‐ODT is able to significantly suppress background phase noise and image cerebral capillary flow down to the vessel size of 5.6 μm. Comparison between the wavelength‐division‐multiplexing SS‐ODT and the spectral‐domain ultrahigh‐resolution ODT (uODT) reveals that SS‐ODT outpaces uODT by extending the capillary flow imaging depth to 1.6 mm in mouse cortex. Thus, for the first time, quantitative capillary flow imaging is demonstrated using SS‐ODT in the deep cortex.