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Comparison between reflectance confocal microscopy and 2‐photon microscopy in early detection of cutaneous radiation injury in a mouse model in vivo
Author(s) -
Jang Won Hyuk,
Kwon Soonjae,
Shim Sehwan,
Jang WonSuk,
Myung Jae Kyung,
Yang Sejung,
Park Sunhoo,
Kim Ki Hean
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700337
Subject(s) - confocal microscopy , in vivo , autofluorescence , microscopy , pathology , confocal , two photon excitation microscopy , preclinical imaging , medicine , biomedical engineering , materials science , biology , optics , microbiology and biotechnology , fluorescence , physics
Cutaneous radiation injury (CRI) is a skin injury caused by high‐dose exposure of ionizing radiation (IR). For proper treatment, early detection of CRI before clinical symptoms is important. Optical microscopic techniques such as reflectance confocal microscopy (RCM) and 2‐photon microscopy (TPM) have been tested as the early diagnosis method by detecting cellular changes. In this study, RCM and TPM were compared in the detection of cellular changes caused by CRI in an in vivo mouse model. CRI was induced on the mouse hindlimb skin with various IR doses and the injured skin regions were imaged longitudinally by both modalities until the onset of clinical symptoms. Both RCM and TPM detected the changes of epidermal cells and sebaceous glands before clinical symptoms in different optical contrasts. RCM detected changes of cell morphology and scattering property based on light reflection. TPM detected detail changes of cellular structures based on autofluorescence of cells. Since both RCM and TPM were sensitive to the early stage CRI by using different contrasts, the optimal method for clinical CRI diagnosis could be either individual methods or their combination.