Premium
Optimization for imaging through scattering media for confocal microscopes with divided elliptical apertures
Author(s) -
Tang Hengjie,
Wu Chenxue,
Gong Wei,
Zheng Yao,
Zhu Xinpei,
Wang Jiahao,
Si Ke
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700293
Subject(s) - optics , confocal , microscope , point spread function , detector , chart , resolution (logic) , physics , confocal microscopy , signal (programming language) , scattering , materials science , computer science , mathematics , artificial intelligence , programming language , statistics
We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal‐to‐background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half‐maximum can be reduced to 4.986 (described in optical coordinates) and SBR can be improved to 0.176. We evaluate this system with the 1951 USAF resolution test chart and the primary cultured neuron from SD rat stained by Map‐2, and observe better imaging performance, which indicates the potential applications in biological science.