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Label‐free imaging and spectroscopy for early detection of cervical cancer
Author(s) -
Jing Yueyue,
Wang Yulan,
Wang Xinyi,
Song Chuan,
Ma Jiong,
Xie Yonghui,
Fei Yiyan,
Zhang Qinghua,
Mi Lan
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700245
Subject(s) - cervical cancer , fluorescence , fluorescence spectroscopy , spectroscopy , cancer , staining , pathology , medicine , chemistry , nuclear magnetic resonance , optics , physics , quantum mechanics
The label‐free imaging and spectroscopy method was studied on cervical unstained tissue sections obtained from 36 patients. The native fluorescence spectra of tissues are analyzed by the optical redox ratio (ORR), which is defined as fluorescence intensity ratio between NADH and FAD, and indicates the metabolism change with the cancer development. The ORRs of normal tissues are consistently higher than those of precancer or cancerous tissues. A criterion line of ORR at 5.0 can be used to discriminate cervical precancer/cancer from normal tissues. The sensitivity and specificity of the native fluorescence spectroscopy method for cervical cancer diagnosis are determined as 100% and 91%. Moreover, the native fluorescence spectroscopy study is much more sensitive on the healthy region of cervical precancer/cancer patients compared with the traditional clinical staining method. The results suggest label‐free imaging and spectroscopy is a fast, highly sensitive and specific method on the detection of cervical cancer.