Premium
Red blood cell membrane damage by light‐induced thermal gradient under optical trap
Author(s) -
Chowdhury Aniket,
Waghmare Deepak,
Dasgupta Raktim,
Majumder Shovan K.
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700222
Subject(s) - membrane , biophysics , electroporation , optical tweezers , red blood cell , cell membrane , temperature gradient , membrane potential , cell damage , chemistry , materials science , optics , biology , biochemistry , physics , quantum mechanics , gene
Rapid membrane damage of optically trapped red blood cells (RBCs) was observed at trapping powers ≥280 mW. An excellent agreement between the estimated laser‐induced thermal gradient across trapped cell's membrane and that typically required for membrane electropermeabilization suggests a mechanism involving temperature gradient‐induced electropermeabilization of membrane. Also the rapid collapse of the trapped cell due to membrane rupture was seen to cause shock waves in the surroundings permeabilizing nearby untrapped cells. When the experiments were carried out with RBCs collected from type II diabetic patients, a noticeable change in the damage rate compared to normal RBCs was seen suggesting a novel optical diagnosis method for the disease.