Premium
Detection of depth‐depend changes in porcine cartilage after wear test using Raman spectroscopy
Author(s) -
Tong Lingying,
Hao Zhixiu,
Wan Chao,
Wen Shizhu
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700217
Subject(s) - cartilage , raman spectroscopy , materials science , biomedical engineering , osteoarthritis , proteoglycan , characterization (materials science) , scanning electron microscope , extracellular matrix , pathology , chemistry , anatomy , composite material , medicine , nanotechnology , optics , biochemistry , physics , alternative medicine
Cartilage damage and wear can lead to severe diseases, such as osteoarthritis, thus, many studies on the cartilage wear process have already been performed to better understand the cartilage wear mechanism. However, most characterization methods focus on the cartilage surface or the total wear extent. With the advantages of high spatial resolution and easy characterization, Raman microspectroscopy was employed for the first time to characterize full‐depth changes in the cartilage extracellular matrix (ECM) after wear test. Sections from the cartilage samples after wear were compared with sections from the control group. Univariate and multivariate analyses both indicated that collagen content loss at certain depths (20%‐30% relative to the cartilage surface) is possibly the dominating alteration during wear rather than changes in collagen fiber orientation or proteoglycan content. These findings are consistent with the observations obtained by scanning electron microscopy and histological staining. This study successfully used Raman microspectroscopy efficiently assess full‐depth changes in cartilage ECM after wear test, thus providing new insight into cartilage damage and wear.