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Correcting the limited view in optical‐resolution photoacoustic microscopy
Author(s) -
Liu Wei,
Zhou Yuan,
Wang Mengran,
Li Lei,
Vienneau Emelina,
Chen Ruimin,
Luo Jianwen,
Xu Chris,
Zhou Qifa,
Wang Lihong V.,
Yao Junjie
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700196
Subject(s) - optics , visibility , reflection (computer programming) , microscopy , lens (geology) , imaging phantom , materials science , signal (programming language) , resolution (logic) , image resolution , photoacoustic tomography , photoacoustic imaging in biomedicine , physics , computer science , artificial intelligence , programming language
Optical‐resolution photoacoustic microscopy (OR‐PAM) has proven useful for anatomical and functional imaging with high spatial resolutions. However, the coherent signal generation and the desired reflection‐mode detection in OR‐PAM can result in a limited detectability of features aligned with the acoustic axis (ie, vertical structures). Here, we investigated the limited‐view phenomenon in OR‐PAM by simulating the generation and propagation of the acoustic pressure waves and determined the key optical parameters affecting the visibility of vertical structures. Proof‐of‐concept numerical experiments were performed with different illumination angles, optical foci and numerical apertures (NA) of the objective lens. The results collectively show that an NA of 0.3 can readily improve the visibility of vertical structures in a typical reflection‐mode OR‐PAM system. This conclusion was confirmed by numerical simulations on the cortical blood vessels in a mouse brain and by experiments in a suture‐cross phantom and in a mouse brain in vivo.