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In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation
Author(s) -
Sherlock Ben,
Warren Sean C.,
Alexandrov Yuriy,
Yu Fei,
Stone James,
Knight Jonathan,
Neil Mark A. A.,
Paterson Carl,
French Paul M. W.,
Dunsby Chris
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700131
Subject(s) - optical coherence tomography , optics , scanner , materials science , speckle pattern , microscope , microscopy , physics
This paper reports a handheld multiphoton fluorescence microscope designed for clinical imaging that incorporates axial motion compensation and lateral image stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Our system is able to correct lateral sample velocities of up to approximately 65 μm s −1 . Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications. The system is used to compensate for both lateral and axial motion of the sample when imaging human skin in vivo.