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Structured illumination microscopy with interleaved reconstruction (SIMILR)
Author(s) -
Ma Ying,
Li Di,
Smith Zachary J.,
Li Dong,
Chu Kaiqin
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700090
Subject(s) - frame rate , microscopy , digital micromirror device , computer science , optics , computer vision , materials science , subframe , super resolution microscopy , resolution (logic) , artificial intelligence , frame (networking) , iterative reconstruction , light sheet fluorescence microscopy , physics , scanning confocal electron microscopy , telecommunications link , computer network , telecommunications
Structured illumination microscopy (SIM) is the commonly used super‐resolution (SR) technique for imaging subcellular dynamics. However, due to its need for multiple illumination patterns, the frame rate is just a fraction of that of conventional microscopy and is thus too slow for fast dynamic studies. A new SR image reconstruction method that maximizes the use of each subframe of the acquisition series is proposed for improving the super‐resolved frame rate by N times for N illumination directions. The method requires no changes in raw data and is appropriate for many versions of SIM setup, including those implementing fast illumination pattern generation mechanism based on spatial light modulator or digital micromirror device. The performance of the proposed method is demonstrated through imaging the highly dynamic endoplasmic reticulum where continuous rapid growths or shape changes of tiny structures are observed.