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Optical properties of mice's stool in 550 to 1000 nm wavelength range
Author(s) -
Isler Helene,
Germanier Catherine,
Ahnen Linda,
Jiang Jingjing,
Lindner Scott,
Di Costanzo Mata Aldo,
Karen Tanja,
Sánchez Majos Salvador,
Wolf Martin,
Kalyanov Alexander
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700076
Subject(s) - mie scattering , rayleigh scattering , wavelength , materials science , optics , scattering , near infrared spectroscopy , absorption (acoustics) , visible spectrum , fluorescence , light scattering , infrared , optoelectronics , physics
The aim of this work was to measure optical properties of stool of mice to provide this relevant wavelength‐dependent behavior for optical imaging modalities such as fluorescent molecular tomography and near‐infrared optical tomography. BALB/c nude female mice were studied and optical properties of the stool were determined by employing the inverse adding‐doubling approach. The animals were kept on chlorophyll‐free diet. Nine stool samples were measured. The wavelength‐dependent behavior of absorption and scattering in 550 to 1000 nm range is presented. The reduced scattering spectrum is fitted to the Mie scattering approximation in the near‐infrared (NIR) wavelength range and to the Mie + Rayleigh approximation in visible/NIR range with the fitting coefficients presented. The study revealed that the absorption spectrum of stool can lead to crosstalk with the spectrum of hemoglobin in the NIR range.