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High‐resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo
Author(s) -
Zhang Pengfei,
Li Lei,
Lin Li,
Hu Peng,
Shi Junhui,
He Yun,
Zhu Liren,
Zhou Yong,
Wang Lihong V.
Publication year - 2018
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700024
Subject(s) - photoacoustic tomography , photoacoustic imaging in biomedicine , biomedical engineering , coronal plane , preclinical imaging , neuroimaging , skull , tomography , transducer , materials science , high resolution , nuclear medicine , in vivo , geology , anatomy , neuroscience , medicine , optics , radiology , physics , biology , remote sensing , acoustics , microbiology and biotechnology
Photoacoustic computed tomography (PACT) is a non‐invasive imaging technique offering high contrast, high resolution, and deep penetration in biological tissues. We report a PACT system equipped with a high frequency linear transducer array for mapping the microvascular network of a whole mouse brain with the skull intact and studying its hemodynamic activities. The linear array was scanned in the coronal plane to collect data from different angles, and full‐view images were synthesized from the limited‐view images in which vessels were only partially revealed. We investigated spontaneous neural activities in the deep brain by monitoring the concentration of hemoglobin in the blood vessels and observed strong interhemispherical correlations between several chosen functional regions, both in the cortical layer and in the deep regions. We also studied neural activities during an epileptic seizure and observed the epileptic wave spreading around the injection site and the wave propagating in the opposite hemisphere.