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3D in vivo imaging with extended‐focus optical coherence microscopy
Author(s) -
Chen Yu,
Trinh Le A.,
Fingler Jeff,
Fraser Scott E.
Publication year - 2017
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201700008
Subject(s) - optical coherence tomography , optics , focus (optics) , depth of focus (tectonics) , microscopy , coherence (philosophical gambling strategy) , biological specimen , preclinical imaging , gaussian beam , optical imaging , resolution (logic) , bessel beam , image resolution , computer science , physics , materials science , bessel function , beam (structure) , artificial intelligence , in vivo , biology , paleontology , subduction , microbiology and biotechnology , quantum mechanics , tectonics
Optical coherence microscopy (OCM) has unique advantages of non‐invasive 3D imaging without the need of exogenous labels for studying biological samples. However, the imaging depth of this technique is limited by the tradeoff between the depth of focus (DOF) and high lateral resolution in Gaussian optics. To overcome this limitation, we have developed an extended‐focus OCM (xf‐OCM) imaging system using quasi‐Bessel beam illumination to extend the DOF to ∼100 μm, about 3‐fold greater than standard OCM. High lateral resolution of 1.6 μm ensured detailed identification of structures within live animal samples. The insensitivity to spherical aberrations strengthened the capability of our xf‐OCM system in 3D biological imaging.